Purpose
This week's lab will be divided into three sections. You will...
- learn how to effectively and reproducibly use a Pipetman with micro-scale liquids;
- measure the concentration of protein in a sample of milk and compare your result to the value from the manufacturer;
- prepare a buffer for use next week.
Objectives
- Demonstrate your ability to use a Pipetman to accurately dispense liquids
- Analyze a written procedure to calculate the amount of chemicals you will need
- Apply a procedure to the preparation standards and samples for reaction-based assays
- Use a UV-Vis spectrophotometer to measure absorbance
- Construct meaning from data collected by plotting a standard curve and determining a milk concentration from it
- Calibrate and use a pH meter
- Apply a written procedure to calculate and prepare a buffer of specified concentration and pH
- Evaluate the quality of a measured value by comparison to an expected value. Professionally present a data figure.
Pre-lab preparation
Read the assigned reading (including this lesson) completely
Complete the prelab assignment at least the day before lab begins
Notebook: State the objectives and write-out the procedures including the tables for each assay.
Lecture Video