Key Concepts
1. Like other cells of the body, neurons at
rest exhibit an electrical potential across the cell membrane.
2. This resting potential is the consequence
of the differential concentrations of ions inside and outside the neuron,
and the semipermeable nature of the membrane. The membrane is primarily
permeable to potassium ions, and when equilibrium is reached between the
diffusion pressure forcing potassium out of the cells and the electrostatic
pressure forcing potassium into the cell, the resting membrane potential
is about ?70 mV.
3. Neurons must actively maintain this ion
balance, due to the gradual leakage of ions across the membrane.
4. Perturbations of the resting potential
that cause it to become more negative, or hyperpolarized, reduce the probability
that a nerve impulse will be produced. Perturbations that depolarize the
membrane increase the probability that a nerve impulse will be produced.
Such perturbations spread passively from the site of stimulation, decreasing
with time and distance.
5. Depolarizations that exceed an individual
neuron’s threshold will cause the production of a nerve impulse, or action
potential, in the axon. The action potential has an amplitude of about
100 mV and is generated in an all-or-none fashion—its size is independent
of stimulus magnitude.
6. For a brief period of time after producing
an action potential, the axonal membrane is completely insensitive to further
stimuli; this is called the absolute refractory phase. This phase is immediately
followed by a brief period of reduced sensitivity called the relative refractory
phase.
7. During the peak of the action potential,
the membrane potential approaches +40 mV, due to sodium permeability that
results from the opening of gated sodium channels. These channels close
rapidly and the resting potential is restored.
8. Gating currents are tiny electrical currents
across the cell membrane that induce voltage-gated channels to open. The
gated sodium channels of the axonal membrane are voltage-gated. These channels
contain an activation gate, which opens as a consequence of depolarization,
and an inactivation gate that closes slowly with depolarization. In the
resting state, the activation gate is closed, and the inactivation gate
is open.
9. Cell bodies and dendrites contain few voltage-gated
ion channels and thus cannot propagate action potentials.
10. The structure and function of ion channels
has been studied using molecular genetic approaches, the use of toxins,
and the patch-clamp technique, in which individual channels may be studied
in isolation.
11. Action potentials arise at the axon hillock
and spread down the axon through a regenerative process at speeds ranging
from 1 to 120 m/s. Conduction velocity is increased in axons with large
diameters, and particularly in axons that are myelinated. In the latter
case, the action potential jumps between nodes of Ranvier; this is called
saltatory conduction.
12. The arrival of an action potential at
the axon terminals of a presynaptic neuron, and the subsequent release
of neurotransmitter, results in a small graded depolarization (excitatory
postsynaptic potential; EPSP) or graded hyperpolarization (inhibitory postsynaptic
potential; IPSP) of the membrane of the postsynaptic cell (depending on
whether the presynaptic cell is excitatory or inhibitory, respectively).
If enough EPSPs are received, the postsynaptic cell’s threshold is reached
and an action potential is produced.
13. The IPSPs and EPSPs produced in a postsynaptic
neuron sum temporally and spatially. If relatively more and/or greater
EPSPs occur, the cell’s threshold is exceeded at the axon hillock and an
action potential is generated. This integration is the basis of information
processing by the neuron.
14. At chemical synapses, the arrival of an
action potential causes voltage-gated calcium channels to open in the membrane
of the axon terminal. Calcium influx induces vesicles to fuse to the synaptic
membrane and release transmitter; the amount released is proportional to
the size of the calcium current.
15. Transmitter diffuses across the synaptic
cleft, binds to receptors, and alters the permeability of the postsynaptic
membrane, resulting in a change in membrane potential. The action of the
transmitter is stopped rapidly, either by chemical inactivation or reuptake.
16. Transmitter receptors operate like a lock
and key; a particular transmitter “key” may open several different types
of receptor “locks” (receptor subtypes).
17. Ionotropic receptors are ligand-gated
ion channels. Metabotropic receptors involve the liberation of chemical
second messengers, which may have numerous effects within the cell.
18. Some synapses are electrical, rather than
chemical, and provide a direct electrical coupling between neurons.
19. Neurons accomplish complex information
processing by acting together in circuits, such as neural chains, feedback
circuits, and oscillator circuits.
20. The electrical activity of populations
of neurons can be measured using scalp electrodes. EEG measures spontaneous
electrical activity, whereas ERPs reflect the reaction of populations of
neurons to discrete stimuli. Both techniques have important diagnostic
applications (such as in epilepsy [EEG] and auditory testing [ERP]), as
well as applications in basic research (such as sleep research [EEG] and
studies of cognitive processing [ERP]).
21. Future research is likely to focus on
combining data on electrical activity with spatial coordinates within the
brain to produce three-dimensional activity maps—this is being accomplished
with techniques such as BEAM, combinations of ERPs and fMRI, and a new
technique that uses light scattering through the skull to detect changes
in blood flow and electrical activity.
22. Under some circumstances, the electrical
activity of large populations of neurons may become synchronized, resulting
in seizures. The behavioral characteristics of the seizures depend on the
site at which the seizures originate and the degree to which the seizure
activity spreads to other regions of the brain.